Next, to determine whether the gene expression pattern from single cells was consistent with the locations of individual tissue cells in the cancer tissue, EA tissue sections were stained with antibodies specific for immune cell markers such as CD4, CD8, TIA1, FOXP3 and CD20, cell proliferation marker such as BIRC5 and MKI67, stromal cell marker such as VIM and ACTA2 (Fig. 2c and Supplementary Fig. 3). The gene discussed is CD4; the disease is cancer.