LGR5 and adenoviridae infectious disease: Single Lgr5-GFP+ cells were sorted from CCL4-treated Lgr5-GFP mice at day 5 after CCL4 treatment and then infected with adenoviral Lgr5 shRNA (Ad-Lgr5 shRNA); both the Lgr5 protein expression and Lgr5 messenger RNA (mRNA) expression were decreased at 72 h after adenovirus infection (Fig. 2a; Supplementary Fig. 8).