To demonstrate whether the Lgr5+ cells numbers, increased by rHGF and rRspo1 combination treatment during liver fibrosis, have liver stem cell properties similar to those of Lgr5+ cells induced by 1XCCL4, single Lgr5+ cells were sorted from Lgr5-GFP mice treated with 1XCCl4 alone or 12XCCl4 (liver fibrosis model) plus rHGF and rRspo1. Here, LGR5 is linked to Hepatic fibrosis.