To further assess whether sCRT could directly suppress differentiation of MDSCs toward DCs, murine splenic G-MDSCs derived from tumor-bearing mice were incubated with GM-CSF for 5 days in the presence of recombinant sCRT39-272 or rEGFP followed by flow cytometric analysis for CD11b+CD11c+ DCs. Here, ITGAX is linked to neoplasm.