To provide direct evidence that KCa3.1 channels participated in ischemic stroke via the ERK1/2 and JNK signaling pathways in vivo, we examined the activation of the ERK1/2 (Figures 8A,B) and JNK (Figures 8A,C) signaling pathways following MCAO blockage for up to 24 h in WT and KO mice. This evidence concerns the gene MAPK3 and ischemic stroke.