Western blot analysis of total proteins from human osteosarcoma cell line lacking endogenous GR (parental U-OFF cells, line 1), transfected with GRα (U-2 OS alpha, line 2) or GRβ (U-2 OS beta, line 3) using different antiGR antibodies is showed in Fig. 1a. This evidence concerns the gene GZMB and osteosarcoma.