To evaluate the relevance of this protein to the actin cytoskeleton in ADPKD cystogenesis, 3-dimensional (3D) culture and F-actin staining were performed after knockdown of either Pkd1 or Pkd2 in mouse inner medullary collecting duct (mIMCD) cells. The gene discussed is PKD1; the disease is autosomal dominant polycystic kidney disease.