The most abundant individual mRNA species were present at one copy per 1000 EVs approximately (e.g., TMSB10), and the levels of mRNAs most commonly mutated in GBM (e.g., EGFR, IDH1, TP53, PTEN, and COL1A2) were not higher than one copy per 100,000 EVs approximately. This evidence concerns the gene COL1A2 and glioblastoma.