Although there was no altered mRNA expression of PPARγ, FGF21, HIF1α, or BECN1 in subcutaneous adipose tissue (supplemental Fig. 3, A–D), expression of the autophagy marker MAP1LC3A was decreased (as indicated by an increased threshold cycle (Ct) value) (p < 0.05) in TRPC1 KO mice fed a HF diet and exercised compared with WT mice fed a HF diet and exercised (Fig. 6A). Here, HIF1A is linked to hydrops fetalis.