To determine whether reduced adipocyte numbers in adipose depots of TRPC1 KO mice fed a HF diet and exercised (Fig. 5) were because of apoptosis or reduced differentiation into adipocytes, we measured mRNA of key markers for adipogenesis (PPARγ (peroxisome proliferator–activated receptor γ)), beiging (FGF21 (fibroblast growth factor 21)), hypoxia (HIF1α (hypoxia-inducible factor 1-α)), and autophagy (MAP1LC3A (microtubule-associated proteins 1A/1B light chain 3A), BECN1 (beclin 1)). This evidence concerns the gene FGF21 and hydrops fetalis.