It was well known that membrane-bound CRT is a danger/eat-me signal and important for immunogenic cell death.25, 26 However, in the current study, AsPC-1 and Capan-2 cells with CRT-GFP exhibited predominantly cytoplasmic green fluorescence in a perinuclear pattern (but not only on cell surface), which is consistent with the studies by Bibi et al.27 and by Ihara et al.28 That might be why high expression of predominantly intracellular CRT executed a pro-tumor role in the development of PC. Here, CALR is linked to pachyonychia congenita.