Moreover, co-staining of CRT with pEGFR1173 (with EGF), Fibronectin and Integrinβ1 and co-immunoprecipitation of CRT with the key proteins of Integrin/EGFR-ERK/MAPK signaling (Fibronectin, Integrinβ1 and c-Myc) were detected in PC cells, which is partially observed in Jurkat cells (CRT/integrin α2β1)46 and atria tissues of patients (CRT/integrinα5 complex).47 In addition, Integrin α1β1 expression is controlled by c-Myc in CRC cells detected by CHIP assays.48 All the above indicated a close interaction of CRT and Integrin/EGFR-ERK/MAPK signal pathway in EGF-induced EMT of PC. This evidence concerns the gene EGFR and pachyonychia congenita.