Furthermore, immunohistochemistry and for paraffin-embedded tissue sections and immunofluorescence for the cell lines showed that FOXO3 was localized in the cytoplasm of cells, and the expression of FOXO3 in normal pancreas tissue and HPDE6 was stronger than in pancreatic cancer cells (Figure 1b). This evidence concerns the gene FOXO3 and familial pancreatic carcinoma.