Furthermore, after demethylzeylasteral injection, mice transplanting with MCL1-overexpressed A375 cells showed bigger tumor volume and bigger tumor weight than mice injecting with empty vector-overexpressed A375 cells.(Figures 7d–f), which means demethylzeylasteral inhibits tumorigenesis in vivo and the inhibition can be retrieved by overexpression of MCL1. Here, MCL1 is linked to neoplasm.