For example, CC can cause endometrial thinning,39 which is not conducive to embryonic implantation, thus leading to decreased pregnancy rates.40 ANP is mostly used for heart and kidney diseases by intravenous infusion during surgical operations.41, 42 In our study, we found that ANP treatment effectively ameliorated most of the symptoms found in PCOS rats, including polycystic ovaries, hyperandrogenism and hormone production, as well as the pregnancy rate. This evidence concerns the gene NPPA and kidney disorder.