PLOD1 and Follicular Cyst: A PCOS animal model is useful for detailed molecular studies of changes in histopathology and hormone synthesis, etc. RU486 could induce the similar features of human PCOS, such as anovulation, enlarged ovaries containing atretic follicles or follicular cysts, as well as increased testosterone, estrogen and LH levels.48 Lakhani et al.49 successfully established the PCOS rat model using RU486, which was used to investigate vascular deficits.