CD8A and neoplasm: To investigate whether functionality of OVA-CD8+ T cells induced by MS-OVA was impaired in B16-OVA tumor bearing mice, we used an in vivo cytotoxicity assay whereby vaccinated mice were injected with CFSE-stained target cells previously pulsed with OVA-peptide and non-pulsed cells as a control and the percentage of in vivo killing was calculated 24 h later based on the survival proportions of target cell populations.