Similarly, the demethylation of hMLH1 promoter gene by DAC and recovery of the mismatch repair system causes the colorectal cancer cells to become sensitive to 5-FU (fluorouracil -5).62 So the combination of epigenetic and conventional chemotherapeutic agents are effective in the treatment of resisted tumors and cancerous cells.63 This evidence concerns the gene MLH1 and colorectal cancer.