In addition, Ki67 staining was performed to investigate the proliferation activity of tumor tissue with RPN2 or EGFR silencing, and our results revealed that the expression level of Ki67 was higher in control mice than in mice inoculated with HCT116-shRPN2 and HCT116-shEGFR (Figure 5F). The gene discussed is MKI67; the disease is neoplasm.