Indeed we establish that (i) MCF-7, MDA-MB-231 and MDA-MB-435S breast cancer cell lines express different levels of IP3R3 protein, (ii) cells expressing IP3R3 in a larger amount migrate more extensively than the others, (iii) silencing of IP3R3 changes a sustained ATP-induced Ca2+ increase to an oscillatory one, (iv) overexpression of IP3R3 increases the migration capacities of the non-invasive MCF-7 cell line and switch the ATP-induced transient Ca2+ response to a sustained phase. This evidence concerns the gene ITPR3 and breast cancer.