More importantly, pAAV-SIRT6 treatment strikingly alleviated Ang II-induced myocardial fibrosis (Figure 8) and ultrastructural injury (Figure 9) in hypertensive rats with activation of phosphorylated AMPK signaling and downregulated levels of fibrosis-related genes CTGF (Figure 6C), FKN (Figure 6D), TGF-b1(Figure 8E), collagen I (Figures 6E, 8F) and collagen III (Figures 6F, 8G). This evidence concerns the gene TGFB1 and Myocardial fibrosis.