YAP promoted the expression of PCNA and Cyclin-D3, whereas 14-3-3ζ inhibited their expression (Figure 7C) Western blot and immunofluorescence assays revealed that 14-3-3ζ overexpression increased the phosphorylation of YAP at the Ser127 site in subcutaneous tumor tissue (Figure 7D and 7F). This evidence concerns the gene CCND3 and neoplasm.