Three bladder cancer cell lines (MB49, UMUC3 and T24) were treated with TFQ or Que with same dose in the range of 0-20 μM for 2 h, and cell lysates were analyzed for their levels of p-AMPK (T172), p-mTOR (S2448) and the mTOR downstream effectors p70S6K and p4EBP1 by Western blot analysis. This evidence concerns the gene RPS6KB1 and urinary bladder cancer.