WNT5A and breast cancer: The breast cancer cell lines used in the present study (MDA-MB-468 and MDA-MB-231) both lack endogenous expression of WNT5A protein, making them ideal to study the effects of WNT5A signaling via either transfection with a WNT5A plasmid or direct stimulation with rWNT5A or Foxy5, a WNT5A-mimic peptide.