Protein lysates from SB-431542 treated MFE-296 tumours showed an expected decrease in Smad phosphorylation, whereas higher p-Smad2 expression was observed in MFE-296 primary tumours (1.9-fold) and metastatic tumours (2.6-fold) compared to Ishikawa tumours at the primary site (Figure 7B). The gene discussed is SMAD2; the disease is metastatic neoplasm.