The positive correlation observed between tGlut and tCho was remarkably similar in both the E1A-Ras transformed cells and all the brain tumour types except oligodendrogliomas (Table 1, S3 Table, Figs 5 and 8B), indicating a strong coupling between glutaminolysis and membrane metabolism of cells on the way to transformation and also in established malignancy. This evidence concerns the gene DHTKD1 and brain neoplasm.