Four major adaptations had to be to done to make this approach feasible for the clinical setting: (1) Decreasing the number of autologous tumor cells, as they are often the limiting factor in the TIL to tumor cell ratio; (2) Up scaling of the coculture assay; (3) Defining the time point of maximal CD137 expression; and (4) Separation of tumor cells from TIL after coculture, as CD137-selected TIL are directly used for large-scale expansion and sequential infusion. This evidence concerns the gene TNFRSF9 and neoplasm.