Here we showed that mouse strains that are susceptible to diet-induced insulin resistance (BL6, 129X1, DBA/2 and FBB/N) all exhibit a HFD-induced reduction in phospholipids that contain a PUFA in both the sn1 and sn2 position, whereas this reduction was not observed in the insulin-sensitive BALB/c mice. Here, INS is linked to Insulin resistance.