The pivotal role of cryopreserved rabbit AM used in this experimental strabismus surgery as a promising biological barrier was corroborated by findings of significantly increased expression of anti-inflammatory cytokines such as TGF-β1 and IL-10 and decreased expression of inflammatory cytokines such as TNF-α, IL-12, and IL-17 in eyes treated with cryopreserved rabbit AM when compared with control eyes. The gene discussed is IL10; the disease is Strabismus.