Further, it is intriguing to note that the Cre-mediated loss of neuronal PrPC did not blunt accumulation of misfolded PrPSc, however PrPSc was reduced in the neurons themselves [153] (accumulations were in supporting cells); thus GOF of prion misfolding was also removed from the neurons during this PrPC ablation, again demonstrating that GOF is required for prion disease progression. This evidence concerns the gene PRNP and prion disease.