NCF4 and bacterial infectious disease: To further determine the functional alterations of p40phox−/− macrophages, peritoneal macrophages from WT and p40phox−/− mice were collected, grown on cover slips, and infected with Salmonella. After incubation in gentamicin-containing medium, we visualized the influence of p40phox deficiency on the uptake and subsequent elimination of Salmonella by macrophages in vitro using immunofluorescent staining approach at 2 and 6 h after bacterial infection.