And patients with N2-N3 of lymph node metastasis (47.36 ± 13.94, 2−ΔΔCt) exhibited an increased serum level of HSP27 mRNA than those with N0-N1 (32.51 ± 8.96, 2−ΔΔCt) (P < 0.001). This evidence concerns the gene HSPB1 and metastatic malignant neoplasm in the lymph nodes.