Once confidence in PD-L1 staining was established in repeated placenta tissues, we compared staining in seven NSCLC patient tissues (patients ‘A’ through ‘G’) with the Ventana SP263 antibody and RK-10-Biotin (Figs 8 and S21–29 When using the RK-10-Biotin peptide we saw heaviest staining localized to the tumor regions of the tissues, which can be very intense based on the concentration of peptide used. Here, CD274 is linked to neoplasm.