Next, we evaluated Daam2 expression in our mouse model of malignant glioma, where we combine in utero electroporation (IUE), with CRISPR-mediated deletion of Nf1, Pten, and Trp53 (herein CRISPR/IUE) (Figure 2—figure supplement 1) (Chen et al., 2016; Chen and LoTurco, 2012; Chen et al., 2012; Cong et al., 2013; John Lin et al., 2017). Here, PTEN is linked to malignant glioma.