Furthermore, we collected the clinical data about HCC patient in our hospital, and found that high expression of DARS2 was related to tumor size (χ2 = 6.084, P = 0.014), HBV infection (χ2 = 24.757, P < 0.0001), BCLC stage (χ2 = 15.245, P = 0.002), cell differentiation (χ2 = 22.667, P < 0.0001), distal metastasis (χ2 = 5.165, P = 0.023), and portal vein invasion (χ2 = 7.813, P = 0.005) using a chi-square test (Table 1). The gene discussed is DARS2; the disease is hepatocellular carcinoma.