Of the 11 proteins that were added to CA125 in the multi-protein classifier, eight of the proteins (CD40.L, CD69, CXCL9, EGFR, EpCAM, SELE, TF, and VEGFR2) have been associated with ovarian cancer in previous studies [29, 79–84]; however, CXCL9 has only been examined in ovarian cancer tissues [85], and CD69 was associated with T-lymphocytes in ovarian cancer ascites fluid [86]. Here, CD40LG is linked to ovarian cancer.