When the classification accuracy of our Proseek® data was evaluated using the ROC curve, 8 of the 12 proteins in our multi-protein classifier had AUC values at or above 0.7 (CA125, CXCL13, CD40L, CD69, LAP-TGF-β1, EpCAM, TF, and EGFR) when tested individually; the levels of three of these proteins (EpCAM, TF, and EFGR) were lower in the early stage ovarian cancer serum samples relative to the healthy controls. The gene discussed is TGFB1; the disease is ovarian cancer.