To evaluate the applicability of LogicTRN in biological systems, we identified regulatory logics formed by ten breast cancer-related TFs ESR1, FOXA1, FOXM1, GATA3, CEBPB, JUN, FOS, JUND, EP300, and CTCF on regulating target genes, by integrating times series gene expression data of the E2-induced tumor progression and ChIP-seq-binding data of these TFs (Supplementary Note 2). This evidence concerns the gene CTCF and breast cancer.