And, in a patient with melanoma, treatment with Imiquimod induced gene expression of angiogenesis or MMP inhibitors, like IFN-α, KiSS1, TIMP metallopeptidase inhibitor 1 (TIMP1), and thrombospondin 1 (THBS1), while decreasing expression of bFGF and MMP9, as shown by quantitative PCR of cutaneous melanoma metastasis biopsies performed before and after treatment [257]. This evidence concerns the gene THBS1 and melanoma.