We used Western blotting analysis to measure the expression levels of APC gene and related genes in the Wnt/β-catenin signaling pathway, including β-catenin, TCF7L1, TCF7L2, LEF1, MMP7, C-myc, C-jun, CYCLIND1 and GSK-3β in both cancer and normal tissues, for the patients who may have the wild or mutation types of the genes, the latter being either heterozygous or homozygous. This evidence concerns the gene JUN and cancer.