Moreover, pGL3-CD47-(−272/+10) but not pGL3-CD47-(−191/+10) displayed greater transcriptional activity in Mel-CV.S than Mel-CV cells, and in melanoma cells from a post-treatment primary culture with increased CD47 expression compared with those from the paired pre-treatment culture (Figure 4D and 4E). Here, CD47 is linked to melanoma.