This includes proteins that have not been previously investigated in BC tissue specimens, such as FUCA1, PGRMC1 and PSMD12 [confirmed by immunohistochemistry (IHC) as detailed above], but also trans-Golgi network integral membrane protein 2 (TGOLN2), transmembrane glycoprotein NMB (GPNMB), and TGFBI. The gene discussed is GPNMB; the disease is breast cancer.