TSC2 and melanoma: In response to PLX treatment up to 3 days in 6 melanoma cell lines, ERK-regulated TSC2 phosphorylation is continuously reduced together with decreased phosphorylation of S6 on serine 235/236, indicating a functional release of TSC2 from ERK inhibition (Figure 4E), whereas the ERK-mediated Raptor phosphorylation is unchanged (data not shown).