Marijuana contains a variety of cannabinoids, of which some, such as cannabidiol and cannabigerol have antagonist properties that may mediate the anti-inflammatory properties of marijuana.[29] In addition, repeated administration of cannabinoids reduced cannabinoid-1 receptor density, which induced a tolerance.[30, 31] Thus, the downregulation of receptor may explain dose-dependent relationship between marijuana use and NAFLD in this study. The gene discussed is CNR1; the disease is metabolic dysfunction-associated steatotic liver disease.