In another study melanoma cell lines with overexpression of CD13/ANPEP, were shown to have reduced expression of melanocytic markers such as gp100, MART-1 and S100B suggestive of a low grade of differentiation.40 In the A375VR4 cell line low expression of SOX10 and MITF and increased expression of CD166 was observed. This evidence concerns the gene ALCAM and melanoma.