Normal intestinal organoids that are cultured in a previously described medium (ENR) will form a budding structure with multi-lineage differentiation, while organoids with Apc depletion will turn into tumor-like “spheroids” that are hyperproliferative and extracellular Wnt signal independent and that lack differentiation (Dow et al., 2015, Drost et al., 2015). Here, APC is linked to neoplasm.