To investigate the role of miR-16 in glioma growth in vitro, we transfected anta-miR-16 into shURGCP-U87 and shURGCP-U251 cells, EdU labeling showed that anta-miR-16 recovered the proliferation activity of URGCP-silenced glioma cells in vitro (Fig. 5e). Here, URGCP is linked to glioma.