In addition, we identified Cyclin D1 and Cyclin E1 as direct targets of miR-16 in glioma cells, overexpressing miR-16 significantly decreased Cyclin D1 and Cyclin E1 expression in glioma cells, suggesting that miR-16 is involved in URGCP-enhanced Cyclin D1 and Cyclin E1 expression in glioma. The gene discussed is CCND1; the disease is glioma.