CCND1 and central nervous system cancer: To investigate whether URGCP regulates the expression of these regulators in glioma, we performed western blotting and found that silencing URGCP only suppressed Cyclin D1 and Cyclin E1 expression, while, URGCP overexpression enhanced Cyclin D1 and Cyclin E1 expression in U87 and U251 cells (Fig. 3a).