To investigate the relationship between miR-16 and Cyclin D1 expression in glioma, we used luciferase assays and found that overexpressing miR-16 reduced luciferase activity; however, binding sit mutation completely abolished the interaction between miR-16 and the 3′UTR of Cyclin D1 (Fig. 5a). The gene discussed is CCND1; the disease is central nervous system cancer.