MYC and glioma: Next, to further confirm c-myc is critical for regulating miR-16 expression in glioma, we silenced c-myc and found that silencing c-myc elevated miR-16 expression in U87 and U251 cells (Supplementary Fig. 3c), while, overexpressing c-myc significantly suppressed miR-16 exprssion in shURGCP-U87 and shURGCP-U251 cells (Fig. 4h).