Such categorisation showed genes with known function in skeletal muscle metabolism that we validated via quantitative PCR (qPCR) such as PPAR γ coactivator 1 α (PGC-1α) and NR4A3 (Supplementary Fig. S2D)22,23, whilst it allowed us to identify a number of novel genes with potential function in the development and treatment of metabolic diseases (Supplementary Fig. S2C). Here, NR4A3 is linked to metabolic disease.