Our results displayed a significant increase in the expressionof GDF-9 in the unfertilized mature oocytes ofPCOS patients after administration of NAC comparedto MET, indicating that NAC, as an anti-oxidant/antiapoptoticagent, could enhance the expression of GDF-9through inhibiting the activity of NF-kB and AP-1 transcriptionfactors, therefore affecting the activity of MAPkinasesignaling and related genes expression (33), whichmay be able to alleviate PCOS follicular disorders andprevent follicular developmental detention and atresia. This evidence concerns the gene NFKB1 and folliculitis.