CFTR and cystic fibrosis: Melis et al. (2014) also demonstrated that the pharmacological inhibitor, CFTR inh-172, is not specific so the small downward shift in vacuolar pH of human and HVCN1−/− mouse neutrophils (Supplementary Table S4) produced by this agent is likely to be due to an off-target effect in the light of the normal results obtained with CF patient cells.