SHH and familial pancreatic carcinoma: In the present study, GDC-0449 was relatively non-toxic to all the five cell lines and did not exhibit synergistic effects with Dox on monocultured BxPC-3, Panc-1, MIAPaca-2, and SW1990 pancreatic cancer cells, thereby indicating that the proliferation of these cell lines is not dependent on SHH signaling.