Studying each individual marker showed that PD-L1–positive membrane expression in malignant cells by chromogenic IHC and mIF (AE1/AE3 plus PD-L1–positive) could be used to identify six positive cases independently of the mIF batch observed, supporting the finding that the PD-L1 (E1L3N) marker used in our study is very efficient for detecting positive PD-L1 tumor cases in a panel of mIF staining. This evidence concerns the gene CD274 and neoplasm.