Using the EdU flow cytometry cell proliferation assay, we found that overexpression of KLF4 with adenovirus (AdKLF4) exhibited a higher percentage of mgR SMCs undergoing replication (8.36%) compared to both no viral infection (mgR) or an empty vector control (AdEmpty) (6.96 and 6.64, respectively). Here, KLF4 is linked to viral infectious disease.