Since phosphorylated FGFR2 is known to be active and capable of recruiting downstream proteins and subsequently activating signaling pathways, we performed a high-throughput reverse phase protein array (RPPA) followed by statistical quantification of the ratio of phosphorylated to total proteins in both HEK 293T cell line and HCC-515 cell line (Supplementary Fig. 3c–e). Here, FGFR2 is linked to hepatocellular carcinoma.