To identify previously unmapped MCM2-responsive phosphorylation proteins and gain system-wide insights into the regulatory role of MCM2 in lung cancer cells, we performed a quantitative phosphoproteomic analysis on 24-h overexpressed-MCM2 (pMCM2) in A549 cells and 48-h silenced-MCM2 (siMCM2) in H1299 cells (Fig. 2a). The gene discussed is MCM2; the disease is lung cancer.